SRA

Disseminated cancer cells reside in the lung alveolar niche where they are exposed to interactions with alveolar macrophages. Here we report singe-cell RNA-sequencing data of lung macrophages from MMTV-HER2 mice at the early stage (16-weeks) harboring single dormant cancer cells and late stage mice (32-weeks harboring metastatic lesions, as well as wildtype control mice. We discovered alveolar macrophage and interstitial macrophage subsets that change in expression profile and frequency between stages. We find majority of the AMs are homeostatic in phenotype, and emergence of inflammatory AM subsets in late-stage MMTV-HER2 mice. Overall design: Lungs from MMTV-HER2 mice and wildtype FVB mice from early stage (16-weeks) and late stage (32-week) were dissociated and macrophages were FACs sorted for single cell RNA sequencing.